Journal: Scientific Reports

Article Title: Depurinized milk downregulates rat thymus MyD88/Akt/p38 function, NF-κB-mediated inflammation, caspase-1 activity but not the endonuclease pathway: in vitro / in vivo study

doi: 10.1038/srep41971

Figure Lengend Snippet: Adult (eight weeks old) female Wistar rats were allocated into three dietary groups (8 rats/group). The first group received depurinized milk (DP milk) instead of standard laboratory chow for 15 days; the second group received only commercial UHT 1.5% cow’s milk instead of standard laboratory chow for 15 days; and the control group received standard laboratory chow. The indirect immunofluorescence assay was performed to measure the quantitative expression of MyD88, Akt-1 kinase, phospho-Akt-1 kinase, p38, phospho-p38 and NF-κB (expressed as the logarithm of fluorescence per quantity of cellular proteins). The activity of acid and alkaline DNase was measured spectrophotometrically based on acid-soluble nucleotide determination after precipitation. Enzyme activity was expressed as U/g protein using corresponding standard commercial DNase I and DNase II.

Article Snippet: All antibodies were purchased from Santa Cruz Biotechnology (USA): MyD88 (B-1 sc-136970), Akt-1 (5c10: sc-81434), phospho-Akt-1 (11E6: sc-81433 mouse monoclonal IgG 1 ), NF-κB (p65 C-20: sc-372 epitope mapping at the C-terminus of NF-κB p65), p38 (p38α/β A-12: sc-7972), phospho-p38 (epitope corresponding to phosphorylated Tyr 182), CD4+ (H-370, sc-7219) and CD8+ (H-160 sc-7188).

Techniques: Control, Immunofluorescence, Expressing, Fluorescence, Activity Assay

Journal: BMC Complementary and Alternative Medicine

Article Title: Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways

doi: 10.1186/1472-6882-14-156

Figure Lengend Snippet: Effects of treatments on protein expression and phosphorylation of p-38 MAPK in the renal tissues of STZ-diabetic rats. STZ-diabetic rats were dosed by oral gavage once per day for eight weeks with 200 mg/kg PSLR (STZ + PSLR 200) or 300 mg/kg PSLR (STZ + PSLR 300). Normal or STZ-diabetic rats receiving vehicle treatment were given the same volume of vehicle (distilled water) used to to disperse PSLR. Ratios of p-p38 MAPK/β-actin, p38 MAPK/β-actin, or p-p38 MAPK/p38 MAPK are expressed as the mean with SD (n = 4 per group) in each column. a P < 0.05 and b P < 0.01 compared to the values of vehicle-treated normal rats (normal + vehicle), respectively. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated STZ-diabetic rats (STZ + vehicle), respectively.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline Tween (20 mmol/l Tris, pH 7.6, 137 mmol/l NaCl, and 0.1% Tween 20) for 3 h at room temperature, followed by an overnight incubation at 4°C with with primary antibodies CD4 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7219), CD8 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7188), nephrin (Santa Cruz Biotechnology, Inc., Cat. No. sc-28192), podocin (Santa Cruz Biotechnology, Inc., Cat. No. sc-21009), NF-κB p65 (Santa Cruz Biotechnology, Inc., Cat. No. sc-109), IκBα (Cell Signaling Technology, Beverly, MA, USA; Cat. No. 9242), p38 MAPK (Cell Signaling Technology; Cat. No. 9212), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology; Cat. No. 9211), or β-actin (Santa Cruz Biotechnology, Inc.; Cat. No. sc-130656).

Techniques: Expressing, Phospho-proteomics

Journal: BMC Complementary and Alternative Medicine

Article Title: Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways

doi: 10.1186/1472-6882-14-156

Figure Lengend Snippet: Effects of treatments on protein expression and phosphorylation of p-38 MAPK in the renal tissues of STZ-diabetic rats. STZ-diabetic rats were dosed by oral gavage once per day for eight weeks with 200 mg/kg PSLR (STZ + PSLR 200) or 300 mg/kg PSLR (STZ + PSLR 300). Normal or STZ-diabetic rats receiving vehicle treatment were given the same volume of vehicle (distilled water) used to to disperse PSLR. Ratios of p-p38 MAPK/β-actin, p38 MAPK/β-actin, or p-p38 MAPK/p38 MAPK are expressed as the mean with SD (n = 4 per group) in each column. a P < 0.05 and b P < 0.01 compared to the values of vehicle-treated normal rats (normal + vehicle), respectively. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated STZ-diabetic rats (STZ + vehicle), respectively.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline Tween (20 mmol/l Tris, pH 7.6, 137 mmol/l NaCl, and 0.1% Tween 20) for 3 h at room temperature, followed by an overnight incubation at 4°C with with primary antibodies CD4 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7219), CD8 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7188), nephrin (Santa Cruz Biotechnology, Inc., Cat. No. sc-28192), podocin (Santa Cruz Biotechnology, Inc., Cat. No. sc-21009), NF-κB p65 (Santa Cruz Biotechnology, Inc., Cat. No. sc-109), IκBα (Cell Signaling Technology, Beverly, MA, USA; Cat. No. 9242), p38 MAPK (Cell Signaling Technology; Cat. No. 9212), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology; Cat. No. 9211), or β-actin (Santa Cruz Biotechnology, Inc.; Cat. No. sc-130656).

Techniques: Expressing, Phospho-proteomics

Journal: BMC Complementary and Alternative Medicine

Article Title: Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways

doi: 10.1186/1472-6882-14-156

Figure Lengend Snippet: Effects of treatments on protein expressions of nephrin and podocin in the renal tissues of STZ-diabetic rats. STZ-diabetic rats were dosed by oral gavage once per day for eight weeks with 200 mg/kg PSLR (STZ + PSLR 200) or 300 mg/kg PSLR (STZ + PSLR 300). Normal or STZ-diabetic rats receiving vehicle treatment were given the same volume of vehicle (distilled water) used to to disperse PSLR. Values (mean ± SD) were obtained for each group of 4 animals. a P < 0.05 and b P < 0.01 compared to the values of vehicle-treated normal rats (normal + vehicle), respectively. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated STZ-diabetic rats (STZ + vehicle), respectively.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline Tween (20 mmol/l Tris, pH 7.6, 137 mmol/l NaCl, and 0.1% Tween 20) for 3 h at room temperature, followed by an overnight incubation at 4°C with with primary antibodies CD4 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7219), CD8 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7188), nephrin (Santa Cruz Biotechnology, Inc., Cat. No. sc-28192), podocin (Santa Cruz Biotechnology, Inc., Cat. No. sc-21009), NF-κB p65 (Santa Cruz Biotechnology, Inc., Cat. No. sc-109), IκBα (Cell Signaling Technology, Beverly, MA, USA; Cat. No. 9242), p38 MAPK (Cell Signaling Technology; Cat. No. 9212), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology; Cat. No. 9211), or β-actin (Santa Cruz Biotechnology, Inc.; Cat. No. sc-130656).

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways

doi: 10.1186/1472-6882-14-156

Figure Lengend Snippet: Effects of treatments on protein expressions of renal IκB and NF-κB in the renal tissues of STZ-diabetic rats. STZ-diabetic rats were dosed by oral gavage once per day for eight weeks with 200 mg/kg PSLR (STZ + PSLR 200) or 300 mg/kg PSLR (STZ + PSLR 300). Normal or STZ-diabetic rats receiving vehicle treatment were given the same volume of vehicle (distilled water) used to to disperse PSLR. Values (mean ± SD) were obtained for each group of 4 animals. a P < 0.05 and b P < 0.01 compared to the values of vehicle-treated normal rats (normal + vehicle), respectively. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated STZ-diabetic rats (STZ + vehicle), respectively.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline Tween (20 mmol/l Tris, pH 7.6, 137 mmol/l NaCl, and 0.1% Tween 20) for 3 h at room temperature, followed by an overnight incubation at 4°C with with primary antibodies CD4 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7219), CD8 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7188), nephrin (Santa Cruz Biotechnology, Inc., Cat. No. sc-28192), podocin (Santa Cruz Biotechnology, Inc., Cat. No. sc-21009), NF-κB p65 (Santa Cruz Biotechnology, Inc., Cat. No. sc-109), IκBα (Cell Signaling Technology, Beverly, MA, USA; Cat. No. 9242), p38 MAPK (Cell Signaling Technology; Cat. No. 9212), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology; Cat. No. 9211), or β-actin (Santa Cruz Biotechnology, Inc.; Cat. No. sc-130656).

Techniques:

Journal: BMC Complementary and Alternative Medicine

Article Title: Polysaccharides from Liriopes Radix ameliorate streptozotocin-induced type I diabetic nephropathy via regulating NF-κB and p38 MAPK signaling pathways

doi: 10.1186/1472-6882-14-156

Figure Lengend Snippet: Effects of treatments on protein expressions of nephrin and podocin in the renal tissues of STZ-diabetic rats. STZ-diabetic rats were dosed by oral gavage once per day for eight weeks with 200 mg/kg PSLR (STZ + PSLR 200) or 300 mg/kg PSLR (STZ + PSLR 300). Normal or STZ-diabetic rats receiving vehicle treatment were given the same volume of vehicle (distilled water) used to to disperse PSLR. Values (mean ± SD) were obtained for each group of 4 animals. a P < 0.05 and b P < 0.01 compared to the values of vehicle-treated normal rats (normal + vehicle), respectively. c P < 0.05 and d P < 0.01 compared to the values of vehicle-treated STZ-diabetic rats (STZ + vehicle), respectively.

Article Snippet: Membranes were blocked with 5% non-fat dry milk in Tris-buffered saline Tween (20 mmol/l Tris, pH 7.6, 137 mmol/l NaCl, and 0.1% Tween 20) for 3 h at room temperature, followed by an overnight incubation at 4°C with with primary antibodies CD4 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7219), CD8 (Santa Cruz Biotechnology, Inc., Cat. No. sc-7188), nephrin (Santa Cruz Biotechnology, Inc., Cat. No. sc-28192), podocin (Santa Cruz Biotechnology, Inc., Cat. No. sc-21009), NF-κB p65 (Santa Cruz Biotechnology, Inc., Cat. No. sc-109), IκBα (Cell Signaling Technology, Beverly, MA, USA; Cat. No. 9242), p38 MAPK (Cell Signaling Technology; Cat. No. 9212), p-p38 MAPK (Thr180/Tyr182) (Cell Signaling Technology; Cat. No. 9211), or β-actin (Santa Cruz Biotechnology, Inc.; Cat. No. sc-130656).

Techniques: